CTGF is a 38kD, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide, and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing, and regulation of extracellular matrix production. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Indirect and Sandwich): 0.5-2ug/ml using 100ul/well antibody solution. Used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant human CTGF. Neutralization: 6-9ug/ml yields one-half maximal inhibition ND50 of the biological activity of human CTGF (5ug/ml). Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human CTGF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity. Note: Applications are based on unconjugated antibody.
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